Product Description
Singclean Procalcitonin (PCT) Test Kit (Fluorescence Immunochromatography) for infection detection
Product Description
Introduction
Procalcitonin (PCT) is the prohormone of calcitonin (CT), which is generally less than 0.1ng/mL in the blood of healthy people, and its secretion increases after being stimulated by pro-inflammatory responses, especially after bacterial infection. Procalcitonin is an important marker that can specifically distinguish bacterial infection from inflammatory response caused by other reasons. Viral infection, allergy, autoimmune disease and transplant rejection do not cause significant elevation of procalcitonin, while local bacterial infection can result in moderate elevation of procalcitonin concentrations. In some cases (neonatal, multiple trauma, burns, major surgery, prolonged or severe cardiogenic shock), the elevation of procalcitonin may not be related to infection and usually returns to normal values quickly.
PCT is an ideal indicator for the auxiliary diagnosis of severe bacterial infection and septicopyemia, sepsis and other diseases, and has high sensitivity and specificity for systemic bacterial infection, septicopyemia, sepsis, etc.
Indications for Use
The test kit is used for the quantitative determination of procalcitonin (PCT) in human serum, plasma or whole blood samples in vitro.
Who Should Have the Procalcitonin (PCT) Test?
Vulnerable groups are infants and newborns, the elderly, patients with a history of serious diseases such as severe infection and severe burns, patients with impaired immune system, and tumor patients. Symptoms are like recurrent chills, high fever, petechiae rash, arthralgia of large joints, hepatomegaly. These people are suggested to have a Procalcitonin (PCT) Test.
Singclean Advantages
Accurate: the fluorescence immunochromatography method based test kit has higher accuracy.
Easier to read: based on fluorescence immunochromatography, easier to read test result than colloidal gold immunochromatography.
Easy for storage: it can be stored at room temperature (4ºC~30ºC).
Longer validity period: 18 months of validity period.
Product Performance Index
a) Accuracy: The recovery rate is between 85% and 115%.
b) Linear range: within the linear range of 0.1ng/mL ~100ng/mL, the linear correlation coefficient r≥0.9900;
c) Blank limit: not higher than 0.05ng/mL;
d) Precision
In-batch precision: The coefficient of variation (CV) is not more than 15%;
Precision between batches: The relative range between batches is not more than 15%.
Product Information
| Product Name | Procalcitonin (PCT) Test Kit |
| Method | Fluorescence immunochromatography |
| Specimen | Whole blood, Plasma, Serum |
| Specification | 10 tests/box, 20 tests/box, 25 tests/box, 30 tests/box, 50 tests/box, 100 tests/box. |
| Components | Each bag contains a test card and a desiccant; the test card is composed of a t shell and a test strip, and the test strip consists of a sample pad, a fluorescent pad (fixed with fluorescently labeled PCT monoclonal antibody 1), nitrocellulose membrane (coated with PCT monoclonal antibody 2 and goat anti-mouse IgG), filter paper and PVC plastic plate |
| Test time | 10 mins |
[Applicable instrument]
Fluorescence immunoassay analyzer
It should be purchased separately and can be used for different Test Items, like CK-MB, IL6, PCT.
For different tests, scan the QR code accordingly.
Specimen Collection
1. Xihu (West Lake) Dis.n serum, plasma or whole blood samples; other body fluids and samples may not give accurate results.
2. Venous blood or fingertip blood should be collected under sterile conditions. It is recommended to use human serum or plasma for testing.
3. Anticoagulation with EDTA, sodium citrate or heparin is recommended for plasma and whole blood samples.
4. After the clinical blood samples are collected, the test must be completed within 4 hours at room temperature; serum and plasma can be stored at 2~8°C for 3 days and stored below -20°C for 5 months. Whole blood samples should not be frozen and stored at 2~8°C for 3 days. Avoid heat inactivating samples, and hemolyzed samples should be discarded.
5. Samples must be returned to room temperature before testing. Cryopreserved samples need to be completely thawed, rewarmed, and evenly mixed before use. Do not CZPT and thaw repeatedly.
Test Procedure:
a) Bring the test kit and sample to be tested to room temperature.
b) Make sure the ID card matches the batch number of the kit, and insert the ID card into the card reading area of the instrument to read the information.
c) Open the inner package of the test card, take out the test card; draw 70μL of serum plasma sample, drop vertically to the test card sampling place, and start timing; draw 70μL whole blood, vertically drop it to the test card sampling place, and immediately add 1 drop whole blood diluent at the sample adding place and start timing.
d) After adding the sample, click “Start Test” on the screen of the fluorescence immunoanalyzer, and the test card will react at room temperature for 10 minutes; insert the test card into the test card slot of the fluorescence immunoanalyzer, and the instrument will automatically test the test card; The test results can be seen on the display screen of the immunoassay analyzer. Click “Print” on the screen to print the results.
Positive Judgment Value or Reference Interval
Reference value: <0.5ng/mL. The reference interval for plasma and whole blood samples is the same as that for serum samples. Due to differences in geography, race, gender and age, it is recommended that each laboratory establish its own positive judgment value or reference interval.
Interpretation of Results
(For reference only, not used as clinical diagnostic criteria, test results need to be combined with other clinical and laboratory data for clinical diagnosis)
| PCT mass concentration (ng/mL) | Clinical significance |
| <0.05 | Normal value |
| <0.5 | No or mild systemic inflammatory response. May be local inflammation or local infection. |
| 0.5-2 | Moderate systemic inflammatory response. Infection may be present. |
| 2-10 | Most likely sepsis, severe sepsis, or septic shock. There is a high risk of organ dysfunction. |
| ≥10 | Almost all severe bacterial sepsis or septic shock. Often accompanied by organ failure, there is a high risk of death. |
Limitation
a) This kit is only for the test of human serum, plasma or whole blood samples. Abnormal hematocrit samples have an impact on the results of the whole blood test. The test results of serum, plasma and whole blood are not significant difference when the hematocrit is between 21% and 48%.
b) The test results need to be combined with other clinical and laboratory data, and if the PCT test results do not match the clinical assessment, further testing is required.
c) False positive results may be caused by: cross-reaction of antibody-like components in the blood; some nonspecific components in blood have similar antigenic determinants to capture fluorescent-labeled antibodies.
d) Hemoglobin, triglycerides and bilirubin in the blood sample can interfere with the test results, where the maximum allowable concentrations are 5g/L, 10g/L and 0.2g/L, respectively.
e) False negative results may be caused by: some unknown components shield the antigenic determinants from binding to the antibody; the unstable PCT antigen gradually degrades with time and temperature and cannot be recognized by the antibody. Valid test results depend on a good reagent and sample storage environment.
f) Other factors may also cause errors in test results, including technical reasons, operational errors and other sample factors.
12. Certificate
CE ISO13485
Detailed Photos
Main Products
Company Profile
| Classification: | Ivd |
|---|---|
| Type: | Ivd |
| Certification: | CE |
| Group: | All Patients with Infection Detection |
| Certificate: | CE |
| Material: | Plastic |
| Samples: |
US$ 0/Piece
1 Piece(Min.Order) | |
|---|
Symptoms of a Broken CV Joint
Whether you have an old or new car, a CV joint is an essential part of the car’s axle. When it breaks, it is important to know the symptoms of a broken CV joint and how to repair it.
Repairing a damaged or torn CV boot
Whether you have a car, truck, SUV or any other type of vehicle, you should regularly check out your CV boot. The CV boot is the first line of defense against dirt and water from entering the axle. If your CV boot is torn, it will allow dirt, water and other debris to get into the joint, causing it to wear out faster. A torn CV boot also allows grease to escape, which can cause damage to the joint.
If you think you might have a torn CV boot, make sure to have it inspected. This should be done at least once a year, although more often if you have an SUV or a vehicle with a lot of mileage. If you notice a torn CV boot or any other type of damage, you should get it fixed right away. It can be expensive to replace an axle, but replacing a boot is much less expensive.
A CV joint is a coupling device that connects the rear wheel of your vehicle to the front wheel. It transfers the torque generated by the engine to the wheels. If it is not lubricated properly, it can wear out and cause expensive repairs. Having the CV boot checked regularly can keep your axle in good working condition and prevent it from wearing out.
You should be able to tell if you have a CV joint that is leaking by the sound it makes when you turn the wheel. The sound will vary with speed, and will be noticeable only at certain speeds. In general, you will hear a rattling sound. You can also get a click or pop from the axle when you turn it. If there is no visible tear, then the axle is fine and will pass inspection.
If you have to replace your CV boot, make sure you do it correctly. To remove the old boot, you should use channel locks or a razor blade to cut it off. You can then use a screwdriver to remove the metal bands. If you want to save time, you can use a cleaning solution to remove dirt and debris from the old boot.
In addition to replacing the boot, you should also check the CV joint for signs of wear. It is important to check the joint for signs of wear because it can cause a rattling noise. The noise can travel to other parts of the chassis and under the vehicle. Also, you may experience strange noises or strange vibrations from the joint, which can make driving difficult or unsafe. If you suspect that your joint is worn out, you should take your vehicle for a drive to see if you can detect any signs of wear.
If you have an older vehicle or one that has been infrequently serviced, you should replace your CV boot at least once a year. It is not hard to do.
Symptoms of a bad CV joint
Symptoms of a bad CV joint aren’t always obvious. The symptoms of a bad CV joint can be hard to spot, and can even be dangerous if they go unnoticed. However, if you know what to look for, you can catch a problem early and save yourself money and trouble. There are many things you can do to find out if your CV joint is faulty, and these include listening for the right sound and performing a visual inspection.
One of the most common symptoms of a bad CV joint is a clicking noise when you turn. The sound is most often heard during sharp turns at low speeds, but it can also be noticed during acceleration or deceleration.
If you hear a clicking noise while you are turning, it’s a good sign that your CV joint isn’t working as well as it should be. This is because a bad CV joint can cause excessive vibrations, which can affect your steering and transmission. Using a jack to lift your vehicle up can also help you determine whether or not your CV joint is faulty.
Another common symptom of a bad CV joint is a loss of alignment. This can be especially dangerous if you are driving on an uneven surface. It can cause your front tires to point in different directions. In addition to the loss of alignment, a bad CV joint can also cause your wheels to bounce around. If you aren’t able to detect a problem quickly, it can lead to a serious accident.
Another symptom of a bad CV joint is the rumbling noise that your wheels make as you change gears. This is especially dangerous when you are driving at speeds of 15 to 25 MPH, as it’s very likely that the joints aren’t properly greased. You should also check to see if your transmission fluid level is low, which can lead to overheating and shifting problems.
The cv joint can also cause a clunking sound when you shift into reverse. This can indicate a faulty inner or outer CV joint, or it can be a sign of a faulty transaxle. If you hear a clunking sound when you’re shifting into reverse, it’s best to have a mechanic inspect it as soon as possible. If you aren’t sure whether or not your cv joint is faulty, or if you don’t know how to repair it, there are many guides to help you. You can also check the area surrounding your CV joint boot for tears or splits.
Other common symptoms of a bad CV joint include a grinding or shuddering sound that occurs when turning, as well as excessive vibrations that can be heard when the car isn’t moving. These noises can be caused by a leak in the transmission fluid, a clogged filter, or a broken shaft seal.
Symptoms of a failed inner CV joint
Symptoms of a failed inner CV joint include clicking noises when turning. These noises may indicate a problem with the CV joint itself or a damaged or worn joint boot. They may also indicate problems with the transmission. When the boot is worn, the CV joint boots may leak grease. This can cause stains to appear around the rim of the wheel. When the boot is damaged, the grease may leak into the wheel well. These leaks can lead to damage to the steering and suspension components.
Another common symptom of a failed inner CV joint is a clunking noise when accelerating or decelerating. This noise is caused by the failure of the joint, which allows for dirt and grease to enter the joint. The noise becomes louder as the acceleration and deceleration speeds increase. The joint must be repaired immediately to avoid damage to the vehicle.
If a clunking sound is heard, the first thing to do is to check the CV joint boot. It should be tightened to the proper torque to prevent damage to the joint. The boot should also be inspected for leaks. If the boot is torn, it may have to be replaced. If there are no leaks, the joint may be in good condition.
If the boot is torn or cracked, it may leak grease inside the wheel well. If the grease leaks, it can be a sign of damage to the joint itself or to the steering and suspension components. Depending on the damage, the repair may involve replacing the entire joint assembly.
If the CV joint fails, the axle will not be able to balance the body of the vehicle. This can cause the vehicle to bounce on flat paved roads. The car may also vibrate and become hard to control. If this happens, you should contact a mechanic to inspect the CV joint.
If you notice a clunking sound or any other symptom of a failed inner CV joint, you should take your vehicle to a mechanic. A mechanic may have a service manual that can be used to diagnose and repair failed CV joints. They will also advise you on the proper procedure for replacing a joint. If the joint has already failed, it can be replaced with a joint banding tool.
Other symptoms of a failed inner CV joint include side-to-side shaking while accelerating. This can occur in any type of vehicle. The clunking sound can also occur when the driver is driving in a circle. A clunking noise is also heard during sharp turns at lower speeds.
If the joint is failing, it may be possible to identify the problem by shifting gears into reverse. This can be done with the brake on. If the transmission fluid is leaking from the CV axle, the shifter will move in reverse instead of forward.
editor by CX 2023-11-15